醫學SCI 論文經典句子

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Jurkat cells cultured in calcium-free RPMI-1640 medium (Gibco BRL; number
22300-107) containing calcium-free 10% FBS were triggered by anti-Fas IgM. The
treated cells were harvested at the indicated time points and incubated with Fluo-3-AM at
a final concentration of 1 micromolar for 30 min at 37°C (Scoltock et al., 2000). The
labeled cells (50,000 cells per treatment) were then analyzed by exciting the cells at 488